Regulatory

Part:BBa_K1431502:Design

Designed by: Yongkang Long, Longze Su   Group: iGEM14_SUSTC-Shenzhen   (2014-10-12)

Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design UAS sequences in plasmids bind to GD5 or TEG, which can bind to Hela cell and transfer the plasmid containing UAS sequences into cell. To enhance plasmid's binding ability of GD5 or TEG, 2*UAS, 5*UAS and 7*UAS were designed.

Source

We refer to the UAS sequence from Winfried Wels's patent Nucleic acid transfer system published in 2002.United States Patent 6498233

References

[http://www.freepatentsonline.com/6498233.html Wels, W., & Fominaya, J. (2002). Nucleic acid transfer system.]